Journal: Oncology Letters

Article Title: Accumulation of stabilin-1 positive macrophages in the early stage of gastric cancer is associated with short cumulative survival

doi: 10.3892/ol.2020.11310

Figure Lengend Snippet: Comparison of number of CD68 + and stabilin-1-positive cells in cancer tissue and remote non-cancerous tissue of primary gastric cancer.

Article Snippet: The following primary antibodies were used: Mouse anti-human CD68 monoclonal antibody (1:50; cat. no. MSK055 KP-1; Zytomed Systems GmbH), rabbit anti-human stabilin-1 polyclonal antibody (2 μg/ml; 1:800; clone number #27606), kindly provided by Professor Julia Kzhyshkowska (Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, University of Heidelberg, Germany), goat anti-human CD163 antibody (1:200; cat. no. 1607; R&D Systems Europe, Ltd.) and goat anti-human SPARC polyclonal antibody (1:60; cat. no. AF941; R&D Systems, Inc.).

Techniques: Comparison

Journal: Oncology Letters

Article Title: Accumulation of stabilin-1 positive macrophages in the early stage of gastric cancer is associated with short cumulative survival

doi: 10.3892/ol.2020.11310

Figure Lengend Snippet: Expression of stabilin-1 by CD68 + macrophages in human gastric adenocarcinoma. Stabilin-1 was detected by rabbit anti-human stabilin-1 polyclonal antibody and Alexa Fluor 488-conjugated donkey anti-rabbit IgG (indicated in green). CD68 was detected by mouse anti-human CD68 monoclonal antibody and Cy3-conjugated donkey anti-mouse IgG (indicated in red). Nuclei were stained with DRAQ5 (indicated in blue). (A) Stabilin-1-negative/CD68 + cells in poorly gastric adenocarcinoma. Scale bar, 25 µm. (B) Stabilin-1-negative/CD68 + and stabilin-1-positive/CD68 + cells in moderately differentiated gastric adenocarcinoma. Scale bar, 25 µm. (C) Stabilin-1-positive/CD68 + cells in well differentiated gastric adenocarcinoma. Scale bar, 25 µm. (D) Typical stabilin-1-positive/CD68 + cell in moderately differentiated adenocarcinoma. Scale bar, 10 µm.

Article Snippet: The following primary antibodies were used: Mouse anti-human CD68 monoclonal antibody (1:50; cat. no. MSK055 KP-1; Zytomed Systems GmbH), rabbit anti-human stabilin-1 polyclonal antibody (2 μg/ml; 1:800; clone number #27606), kindly provided by Professor Julia Kzhyshkowska (Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, University of Heidelberg, Germany), goat anti-human CD163 antibody (1:200; cat. no. 1607; R&D Systems Europe, Ltd.) and goat anti-human SPARC polyclonal antibody (1:60; cat. no. AF941; R&D Systems, Inc.).

Techniques: Expressing, Staining

Journal: Oncology Letters

Article Title: Accumulation of stabilin-1 positive macrophages in the early stage of gastric cancer is associated with short cumulative survival

doi: 10.3892/ol.2020.11310

Figure Lengend Snippet: Expression of stabilin-1 by CD68 + /CD163 + macrophages in human gastric adenocarcinoma demonstrated by immunofluorescence staining. Stabilin-1 was detected by rabbit anti-human stabilin-1 polyclonal antibody and Alexa Fluor 488-conjugated donkey anti-rabbit IgG (indicated in green). CD163 was detected by goat anti-human CD163 antibody Cy3-conjugated donkey anti-goat IgG (indicated in red). CD68 was detected by mouse anti-human CD68 monoclonal antibody and Alexa Fluor 647-conjugated donkey anti-mouse IgG (indicated in blue). (A) CD68 + /CD163 + /stabilin-1- negative macrophages in gastric adenocarcinoma. Scale bar, 25 µm. (B) CD68 + /CD163 low /stabilin-1-positive macrophages in gastric adenocarcinoma. Scale bar, 25 µm. (C) CD68 + /CD163 low /stabilin-1-positive and CD68 + /CD163 + /stabilin-1-positive cells in gastric adenocarcinoma. Scale bar, 25 µm. (D) Single CD68 + /CD163 + /stabilin-1-positive cell in gastric adenocarcinoma. Scale bar, 2.5 µm.

Article Snippet: The following primary antibodies were used: Mouse anti-human CD68 monoclonal antibody (1:50; cat. no. MSK055 KP-1; Zytomed Systems GmbH), rabbit anti-human stabilin-1 polyclonal antibody (2 μg/ml; 1:800; clone number #27606), kindly provided by Professor Julia Kzhyshkowska (Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, University of Heidelberg, Germany), goat anti-human CD163 antibody (1:200; cat. no. 1607; R&D Systems Europe, Ltd.) and goat anti-human SPARC polyclonal antibody (1:60; cat. no. AF941; R&D Systems, Inc.).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Oncology Letters

Article Title: Accumulation of stabilin-1 positive macrophages in the early stage of gastric cancer is associated with short cumulative survival

doi: 10.3892/ol.2020.11310

Figure Lengend Snippet: Association of stabilin-1 and CD68 expression in TNM stage I gastric cancer with cumulative patient survival. Kaplan-Meier analysis and a log rank test were used to compare patient survival with low and high numbers of intratumoral stabilin-1-positive and CD68 + cells. (A) The association of stabilin-1 expression with patient survival. (B) The association of CD68 expression with patient survival. The cutoff value for determining CD68 and stabilin-1 expression as low and high expression was 16.2 cells and 8.0 cells, respectively. TNM, Tumor-Node-Metastasis; Cum, cumulative.

Article Snippet: The following primary antibodies were used: Mouse anti-human CD68 monoclonal antibody (1:50; cat. no. MSK055 KP-1; Zytomed Systems GmbH), rabbit anti-human stabilin-1 polyclonal antibody (2 μg/ml; 1:800; clone number #27606), kindly provided by Professor Julia Kzhyshkowska (Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, University of Heidelberg, Germany), goat anti-human CD163 antibody (1:200; cat. no. 1607; R&D Systems Europe, Ltd.) and goat anti-human SPARC polyclonal antibody (1:60; cat. no. AF941; R&D Systems, Inc.).

Techniques: Expressing